Sampling of Mediterranean fan mussel specimens (Pinna nobilis) for molecular analyses of the byssus and of the shell matrix (2021)
Data creator :
Frédéric Marin [1] [2]
Project member :
Sébastien Motreuil [1] [2],
Delphine Pasche [3]
Related person :
Alain Garcia [4],
Laurent Gilletta de Saint-Joseph [4],
David Luquet [4],
Chantal Dumas [4]
[1] : Laboratoire Biogéosciences (UMR 6282) (Université de Bourgogne)
[2] : Observatoire des Sciences de l'Univers - Terre, Homme, Environnement, Temps, Astronomie (UAR 3245) (Université de Bourgogne)
[3] : Max Planck Institute of Colloids and Interfaces (Potsdam)
[4] : Laboratoire d'océanographie de Villefranche (UMR 7093)
Description :
This document describes a field mission carried out in May 2017, at the Observatoire Océanologique de Villefranche-sur-Mer. This mission aimed at collecting live specimens as well as dead shells from the Mediterranean fan mussel, Pinna nobilis, an endemic species protected by European Directive 92/43 / EEC.
After obtaining the necessary authorizations from the DDTM of the Alpes Maritimes, the mission consisted in collecting 3 types of samples, via underwater dives in the Bay of Villefranche-sur-Mer and in the surrounding of the Lérins Islands:
- Byssus of live specimens, collected on site, without any displacement nor damage of the specimens.
- Shells of dead specimens, juvenile and adult.
- Live juvenile specimens, which have been acclimated in the basin for several days before sacrifice and removal of calcifying mantles, byssus glands and various organs for subsequent molecular analyses (high thoughput transcriptome sequencing)
After obtaining the necessary authorizations from the DDTM of the Alpes Maritimes, the mission consisted in collecting 3 types of samples, via underwater dives in the Bay of Villefranche-sur-Mer and in the surrounding of the Lérins Islands:
- Byssus of live specimens, collected on site, without any displacement nor damage of the specimens.
- Shells of dead specimens, juvenile and adult.
- Live juvenile specimens, which have been acclimated in the basin for several days before sacrifice and removal of calcifying mantles, byssus glands and various organs for subsequent molecular analyses (high thoughput transcriptome sequencing)
Disciplines :
biodiversity conservation (applied biology - ecology), biology (applied biology - ecology), biology, miscellaneous (applied biology - ecology), materials science, composites (chemistry), materials science, multidisciplinary (chemistry), nanoscience & nanotechnology (chemistry), biochemistry & molecular biology (fundamental biology), cell biology (fundamental biology), materials science, biomaterials (fundamental biology), medical informatics (fundamental biology), microscopy (fundamental biology), environmental sciences (sciences of the universe), marine & freshwater biology (sciences of the universe), multidisciplinary sciences
Keywords :
Access details :
To access the data, please contact the scientific data manager.
General metadata
Data acquisition date :
from 8 May 2017 to 16 May 2017
Data acquisition methods :
- Observational data : Plongée entre 5 et 12 mètres
Collecte de byssus in situ.
Collecte d'individus vivants et acclimatation en aquarium.
Formats :
application/msword, application/pdf, application/vnd.ms-excel, image/jpeg, video/mp4
Audience :
Middle school Education, Community College, University: licence, University: master, Research, Policy maker
Coverages
Spatial coverage :
- Plateau du Milieu (entre Iles de Lérins) et côte Nord Ile Ste-Marguerite: latitude between 43° 30' 59" N and 43° 30' 34" N, longitude between 7° 2' 21" E and 7° 3' 19" E
- Rade de Villefranche-sur-Mer, en face La Darse: latitude between 43° 41' 48" N and 43° 41' 34" N, longitude between 7° 18' 30" E and 7° 19' 6" E
Taxonomic coverage :
- Espèce étudiée. Pinna nobilis LInnaeus 1758 est un bivalve ptériomorphe à microstructure coquillière nacro-prismatique, endémique de la mer Méditerranée. Embranchement: MOLLUSCA. Classe: BIVALVIA. Sous-classe: Pteriomorphia. Ordre: Ostreida. Superfamille: Pinnoidea. Famille: Pinnidae. Genre: Pinna. Espèce: nobilis.SpeciesPinna nobilis
(Mediterranean fan mussel)
Publications :
- A new twist on sea silk: the peculiar protein ultrastructure of fan shell and pearl oyster byssus. (doi:10.1039/c8sm00821c)
Publisher :
Laboratoire Biogéosciences (UMR 6282)
Project and funders :
- Pinna nobilis, source de macromolécules pour matériaux biomimétiques
- Aide logistique de l'EMBRC (European Marine Biology Resource Center) (Another international)
- SRO OSU THETA 2017 (OSU)
Additional information :
Arrêté préfectoral n° 2017-459 portant autorisation de capture d'une espèce de bivalve protégé (Pinna nobilis - Grande Nacre), signé le 04 Mai 2017 par le Directeur de la DDTM, Monsieur Serge CASTEL, pour le Préfet et par délégation.
Record created 11 Apr 2019 by Frédéric Marin.
Local identifier: FR-18008901306731-2019-04-11.
