Abondances of gram-negative bacteria in the environment - floodplains of the Ain, Doubs, and Loue Rivers - 2015-2016 (2021)
Data creators :
Daniel Martak [1] [2],
Didier Hocquet [1] [2],
Charles Henriot [1] [2],
Gudrun Bornette [1] [2]
Data collector :
Quentin Cuenot [1] [2]
[1] : Laboratoire Chrono-environnement (UMR 6249) (Université de Franche-Comté)
[2] : Observatoire des Sciences de l'Univers - Terre, Homme, Environnement, Temps, Astronomie (UAR 3245) (Université de Franche-Comté)
Description :
19 sites (16 wetlands and 3 rivers) with different physicochemical characteristics and distributed along three rivers (Ain, Doubs, and Loue) were sampled. The abundances of some gram-negative bacteria (Escherichia coli, extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa) were determined.
Disciplines :
Keywords :
General metadata
Data acquisition date :
from Apr 2015 to Mar 2016
Data acquisition methods :
- Observational data : Samples of water (250 ml) were collected in sterile conditions and stored at 4◦C before analysis, which occurred within 8 h of collection. The total concentrations of E. coli, K. pneumoniae and
P. aeruginosa were measured as previously described (Slekovec et al. 2012; Brechet ´ et al. 2014). Aliquots of water (100 ml) were filtered through 0.45-μm membranes, which were then placed onto a Drigalski agar plate to measure the total E. coli and K. pneumoniae concentrations (Oxoid), a Cetrimide agar plate to measure the P. aeruginosa concentration (Bio-Rad) and a chromID ESBL agar plate to measure the concentration of E. coli producing extended-spectrum beta-lactamases (ESBL), which are resistant to β-lactam antibiotics. When we suspected high bacterial concentrations, we plated 500 μl an additional of sample on the selective media. The plates were incubated for 24 h at 37◦C. Colonies were counted and classified according to their phenotype. Each type of colony was identified using a MALDITOF MS spectrometer (Microflex, Bruker Daltonics, Bremen, Germany) according to the manufacturer’s recommendations. For each morphotype of E. coli growing on chromID ESBL, the production of ESBL was detected using a synergy test following the recommendations of the Antibiogram Committee of the French Society for Microbiology (CA-SFM, http://www.sfm-microbiologie.org/, last consultation: 10 November 2018).
Formats :
application/vnd.ms-excel, application/vnd.oasis.opendocument.spreadsheet, text/csv
Audience :
University: licence, University: master, Research
Coverages
Spatial coverage :
19 entries
Taxonomic coverage :
- Bactéries étudiéesSpeciesEscherichia coli
(Escherichia coli), Klebsiella pneumoniae (Klebsiella pneumoniae), Pseudomonas aeruginosa (Pseudomonas aeruginosa)
Publications :
- Occurrence and ecological determinants of the contamination of floodplain wetlands with Klebsiella pneumoniae and pathogenic or antibiotic-resistant Escherichia coli (doi:10.1093/femsec/fiz097)
- Déterminismes environnementaux de la diffusion et de la survie des bacilles à Gram négatif commensaux et pathogènes dans les hydrosystèmes (hal:tel-03643429)
Publisher :
Laboratoire Chrono-environnement (UMR 6249)
Label :
Plateforme UBFC
Projects and funders :
-
Thèse de doctorat en microbiologie environnementale - Charles Henriot
- Contrat doctoral Ministère de l'Enseignement Supérieur, de la Recherche et de l'Innovation (MESRI) (Ministry)
-
Fonctionnement des zones humides et risque sanitaire dans le contexte du changement global
- Structuring Initiative Continental and Coastal Ecosphere program (EC2CO) - INSU (CNRS)
Additional information :
Data acquired during Charles Henriot's PhD work, supervised by Pr. Didier Hocquet (University Professor - Hospital practitioner at the Chrono-environment laboratory) and Dr. Gudrun Bornette (Researcher in ecology at the Chrono-environment laboratory)
DOI and links
10.25666/DATAOSU-2021-10-22-03
https://dx.doi.org/doi:10.25666/DATAOSU-2021-10-22-03
https://search-data.ubfc.fr/FR-18008901306731-2021-10-22-03
Quotation
Daniel Martak, Didier Hocquet, Charles Henriot, Gudrun Bornette (2021): Abondances of gram-negative bacteria in the environment - floodplains of the Ain, Doubs, and Loue Rivers - 2015-2016. Chrono-environnement. doi:10.25666/DATAOSU-2021-10-22-03
Record created 22 Oct 2021 by Charles Henriot.
Last modification : 7 Apr 2023.
Local identifier: FR-18008901306731-2021-10-22-03.
