Raman spectra of extracellular vesicles derived from HMEC-1 cells exposed or not to B[a]P (2024) (2024)

Data creator : Benjamin Brunel [1]
[1] : Franche-Comté Electronique Mécanique Thermique et Optique - Sciences et Technologies (UMR 6174) (Université de Franche-Comté)
[2] : Institut de Recherche en Santé Environnement et Travail
[3] : Laboratoire Matière et Systèmes Complexes
[4] : Department of Chemistry, Graduate School of Science and Technology, Kwansei Gakuin University
[5] : Laboratoire Interdisciplinaire Carnot de Bourgogne (UMR 6303) (Université de Bourgogne)
Description :
Raman spectra (693) of extracellular vesicles derived from HMEC-1 cells exposed or not to Benzo[a]pyrene (B[a]P). Data were collected with a Raman microscope on 2 samples exposed to B[a]P and 3 samples control, and preprocessed (baseline correction, normalization). Further details can be found in a publication currently being submitted (link to be added later).
Disciplines :
cell biology (fundamental biology), spectroscopy (physics)
Access details :
To access the data, please contact the scientific data manager.

General metadata

Data acquisition date : from Apr 2023 to Jun 2023
Data acquisition methods :
  • Experimental data :
    This dataset comprises 693 Raman spectra, themselves comprising 1531 decimal numbers (arbitrary units). Two vectors are added: one of 1531 decimal numbers (from 449.6 to 3400.1 cm-1) for the Raman shift and one of 693 character strings for the labels.
    Raman spectra were measured on extracellular vesicles (EVs) from HMEC-1 cells, adsorbed onto a gold chip. Some cells were exposed to a poly-aromatic hydrocarbon: benzo[a]pyrene (B[a]P). Data were taken at several ROIs (3 um steps) on the different biochips, 3 biochips with EVs from exposed cells (“BaP” label) and 2 biochips with EVs from unexposed cells (“control” label). A 532 nm laser was used, with a power of 10 mW and an exposure time of 200 ms, with 50 accumulations.
    Spectra were pre-processed, removing the baseline by arPLS, and normalizing to have a mean intensity of zero between 1900 and 2500 cm-1, and a mean intensity equal to 1 between 2835 and 2970 cm-1.
    Further details can be found in a publication currently being submitted (link to be added later).
Update periodicity : no update
Language : English (eng)
Formats : text/csv
Audience : Research
Projects and funders :

DOI and links

10.25666/DATAUBFC-2024-07-19
https://dx.doi.org/doi:10.25666/DATAUBFC-2024-07-19
https://search-data.ubfc.fr/FR-13002091000019-2024-07-19

Quotation

Benjamin Brunel (2024): Raman spectra of extracellular vesicles derived from HMEC-1 cells exposed or not to B[a]P (2024). dataUBFC. doi:10.25666/DATAUBFC-2024-07-19

Record created 19 Jul 2024 by Benjamin Brunel.
Local identifier: FR-13002091000019-2024-07-19.

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Université de Bourgogne, Université de Franche-Comté, UTBM, AgroSup Dijon, ENSMM, BSB, Arts des Metiers