Raman spectra of extracellular vesicles derived from HMEC-1 cells exposed or not to B[a]P (2024) (2024)
Data creator :
Benjamin Brunel [1]
Project member :
Geetika Raizada [1],
Joan Guillouzouic [2],
Kelly Aubertin [3],
Shinsuke Shigeto [4],
Yuka Nishigaki [4],
Eric Lesniewska [5],
Eric Le Ferrec [2],
Wilfrid Boireau [1],
Céline Elie-Caille [1]
[1] : Franche-Comté Electronique Mécanique Thermique et Optique - Sciences et Technologies (UMR 6174) (Université de Franche-Comté)
[2] : Institut de Recherche en Santé Environnement et Travail
[3] : Laboratoire Matière et Systèmes Complexes
[4] : Department of Chemistry, Graduate School of Science and Technology, Kwansei Gakuin University
[5] : Laboratoire Interdisciplinaire Carnot de Bourgogne (UMR 6303) (Université de Bourgogne)
Description :
Raman spectra (693) of extracellular vesicles derived from HMEC-1 cells exposed or not to Benzo[a]pyrene (B[a]P). Data were collected with a Raman microscope on 2 samples exposed to B[a]P and 3 samples control, and preprocessed (baseline correction, normalization). Further details can be found in a publication currently being submitted (link to be added later).
Disciplines :
Access details :
To access the data, please contact the scientific data manager.
General metadata
Data acquisition date :
from Apr 2023 to Jun 2023
Data acquisition methods :
- Experimental data : This dataset comprises 693 Raman spectra, themselves comprising 1531 decimal numbers (arbitrary units). Two vectors are added: one of 1531 decimal numbers (from 449.6 to 3400.1 cm-1) for the Raman shift and one of 693 character strings for the labels.
Raman spectra were measured on extracellular vesicles (EVs) from HMEC-1 cells, adsorbed onto a gold chip. Some cells were exposed to a poly-aromatic hydrocarbon: benzo[a]pyrene (B[a]P). Data were taken at several ROIs (3 um steps) on the different biochips, 3 biochips with EVs from exposed cells (“BaP” label) and 2 biochips with EVs from unexposed cells (“control” label). A 532 nm laser was used, with a power of 10 mW and an exposure time of 200 ms, with 50 accumulations.
Spectra were pre-processed, removing the baseline by arPLS, and normalizing to have a mean intensity of zero between 1900 and 2500 cm-1, and a mean intensity equal to 1 between 2835 and 2970 cm-1.
Further details can be found in a publication currently being submitted (link to be added later).
Formats :
text/csv
Audience :
Research
Projects and funders :
-
IdEx Université Paris Cité
- ANR-18-IDEX-0001 (French National Agency for Research)
-
SESAME 2019 – IVETh (EX047011)
- SESAME 2019 – IVETh (EX047011) (Another region)
-
France 2030 “Integrateur biotherapie-bioproduction”
- ANR-22-AIBB-0002 (French National Agency for Research)
-
EIPHI - NOVICE
- ANR-17-EURE-0002 (French National Agency for Research)
-
ENDOMITOPAH
- ANR R23137NN (French National Agency for Research)
DOI and links
10.25666/DATAUBFC-2024-07-19
https://dx.doi.org/doi:10.25666/DATAUBFC-2024-07-19
https://search-data.ubfc.fr/FR-13002091000019-2024-07-19
Quotation
Benjamin Brunel (2024): Raman spectra of extracellular vesicles derived from HMEC-1 cells exposed or not to B[a]P (2024). dataUBFC. doi:10.25666/DATAUBFC-2024-07-19
Record created 19 Jul 2024 by Benjamin Brunel.
Local identifier: FR-13002091000019-2024-07-19.
